SCF Protein, Mouse, Recombinant (ECD, His Tag)
產(chǎn)品名稱: SCF Protein, Mouse, Recombinant (ECD, His Tag)
產(chǎn)品型號(hào): 50487-M08B?
產(chǎn)品展商: 義翹
產(chǎn)品文檔: 無相關(guān)文檔
簡(jiǎn)單介紹
產(chǎn)品名稱：SCF Protein, Mouse, Recombinant (ECD, His Tag)
包裝：5ug
SCF Protein, Mouse, Recombinant (ECD, His Tag)
的詳細(xì)介紹
SCF Protein, Mouse, Recombinant (ECD, His Tag): 產(chǎn)品信息
純度
> 95 % as determined by SDS-PAGE
內(nèi)**
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1. Measured by its binding ability in a functional ELISA. Immobilized mouse KITL at 2 μg/ml (100 μl/well) can bind mouse CD117 with a linear range of 1.28-32 ng/ml.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 5-30 ng/mL.
蛋白構(gòu)建
A DNA sequence encoding the amino acids (Met 1-Ala 189) of mouse KITL (P20826-1) was fused with a polyhistidine tag at the C-terminus.
表達(dá)宿主
Baculovirus-Insect Cells
分子量
The secreted recombinant mouse KITL consists of 175 amino acids and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.
緩沖液
Lyophilized from sterile 20mM Tris, 50mM NaCl, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
運(yùn)輸方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
穩(wěn)定性 & 儲(chǔ)存條件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
復(fù)溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
